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Hi. I'm Brian and I'll try to help.
When you change the motherboard, you change your computer. Windows is licensed for a single computer only. Your computer is no longer that original computer and so the license doesn't match the hardware. See if you can reactivate it using the guidance here: https://support.microsoft.com/windows/activation/reactivating-windows-after-a-hardware-change. If you can't, you'll need a new license.