Hola Maheswaran,
What you're seeing aligns with a documented Windows Server 2025 printing compatibility change, although your exact UNIDRVUI.DLL error isn't explicitly called out in the current known issues list.
Starting with the September 2025 Windows Server 2025 update, Microsoft changed parts of the Windows printing stack from the older MSVCRT runtime to the Universal C Runtime. Microsoft explicitly states that older Windows Print clients can intentionally fail when connecting to an updated Windows Server 2025 print server. Microsoft documents the change here.
The important part is beside the Print items. Microsoft says clients older than Windows 10 version 2004 / Windows Server version 2004 aren't compatible with this updated remote-printing path, and their supported workaround is either to upgrade the client or have those older clients use a pre-Windows Server 2025 print server.
That makes Server 2016 seem like a problem here. For the Windows 10 machines, I'd check their exact version/build before assuming they're affected for the same reason. If they're older than the supported level, that would fit Microsoft's documented behavior.
Your observation that the 26100 core package comes back after reconnecting also makes sense with how package-aware Type 3 drivers work. Unidrv is a Windows core print driver. During Point and Print, Windows can install the core driver package required by the printer driver, and Microsoft documents that the installer selects the newest applicable core driver package from the driver store.
So I wouldn't try to pin or manually restore the older UNIDRV files on the client. As you've already found, Point and Print just reverts the newer dependency, and manually mixing core print files from different Windows versions isn't a supported fix.
I also couldn't find a Microsoft-documented switch that tells a Server 2025 print server to keep serving an older Unidrv core package to legacy clients. Microsoft's documented compatibility guidance points the other direction: upgrade the clients, or keep a pre-Server 2025 print server available for older systems.
One thing I'd verify before calling the Server 2019 result definitive is whether that machine already had the printer/driver installed before the Server 2025 migration. Since Server 2019 is also older than build 19041, a fresh connection from a clean Server 2019 client would be a useful comparison.
Thanks,
James