Hi Yashpal Bhandari,
Since the VLAN works when an IP address is assigned directly to the physical network adapter, the physical switch configuration is likely correct and the VLAN traffic is reaching the host. This suggests that the issue may be occurring somewhere between the Hyper-V virtual switch and the physical adapter stack on this particular node.
A few things I would check on the affected server:
1. Verify the switch port configuration for all team members
If the virtual switch is using Switch Embedded Teaming (SET) or an LBFO team, make sure every physical switch port connected to this host is configured identically. I've seen situations where one uplink was missing a VLAN from its allowed trunk list. In that case, traffic can work intermittently or fail completely depending on which uplink is being used.
2. Compare NIC drivers and firmware with a working node
Since the other four cluster nodes are functioning correctly, compare the NIC driver and firmware versions on the affected server against one of the healthy nodes. Differences in drivers or firmware can sometimes cause VLAN-tagged traffic to behave unexpectedly even when host-level connectivity appears normal.
3. Test with VMQ and hardware offloads disabled
VMQ and certain NIC offload features have occasionally been involved in networking issues affecting virtual machines. As a test, try temporarily disabling VMQ on the physical adapters backing the virtual switch:
Disable-NetAdapterVmq -Name "<PhysicalAdapterName>"
If connectivity starts working afterward, it will point toward a driver, firmware, or hardware offload issue.
4. Compare Hyper-V virtual switch settings
It may also be worth comparing the virtual switch configuration on the affected node with one of the working servers:
Get-VMSwitch | Format-List *
Also verify that the VM connected to the affected network is configured for the correct VLAN:
Get-VMNetworkAdapterVlan -VMName "<AffectedVM>"
Finally, check whether any third-party virtual switch extensions or filter drivers are installed on this host but not on the others, as they can sometimes interfere with VLAN-tagged traffic.
Given that all other cluster nodes are working and the VLAN can be reached directly from the host, I would focus first on the physical uplink configuration for all team members and then compare the NIC driver/firmware versions against a known good node. Those are the most likely places where I would expect to find a difference.
If this helps resolve your problem, please consider hitting "Accept Answer" so other users facing this failure can easily find the solution!